Concerns About Unreliable Data from Spotted cDNA Microarrays Due to Cross-Hybridization and Sequence Errors

Daniel Handley, University of Pittsburgh
Nicoleta Serban, Carnegie Mellon University
David G. Peters, University of Pittsburgh
Clark Glymour, Carnegie Mellon University

Abstract

We discuss our concerns regarding the reliability of data generated by spotted cDNA microarrays. Two types of error we highlight are cross-hybridization artifact due to sequence homologies and sequence errors in the cDNA used for spotting on microarrays. We feel that statisticians who analyze microarray data should be aware of these sources of unreliability intrinsic to cDNA microarray design and use.

Submitted: August 23, 2004 · Accepted: September 17, 2004 · Published: October 6, 2004

Recommended Citation

Handley, Daniel; Serban, Nicoleta; Peters, David G.; and Glymour, Clark (2004) "Concerns About Unreliable Data from Spotted cDNA Microarrays Due to Cross-Hybridization and Sequence Errors," Statistical Applications in Genetics and Molecular Biology: Vol. 3 : Iss. 1, Article 25.
Available at: http://www.bepress.com/sagmb/vol3/iss1/art25

 
 
 
 

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