Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models

Durairaj Ashokkumar, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
Upal Kanti Mazumder, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
Malaya Gupta, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
GP Senthilkumar, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India
Vaiyapuri Thamil Selvan, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India

Abstract

Objective - The present study was aimed to evaluate the antioxidant and free radical scavenging properties of Oxystelma esculentum (MEOE).

Methods- The total antioxidant, reducing power, DPPH radical, superoxide anion radical, nitric oxide radical, hydroxyl radical, hydrogen peroxide scavenging and total phenol content in MEOE was carried out.

Results - The total antioxidant activity of MEOE increases with increasing concentration. The reducing capability and free radical scavenging activity in DPPH also increased in a dose dependent manner. The MEOE was found to scavenge the superoxide generated by PMS/NADH/NBT system. Moreover, MEOE was found to inhibit the nitric oxide radical generated from sodium nitropruside. MEOE was also found to inhibit the hydroxyl radical generated by Fe3+/ascorbate/EDTA/H2O2 system. The MEOE scavenges the hydrogen peroxide in a dose dependent manner. The amount of total phenolic compounds was also determined.

Conclusion - The results suggest that the methanol extract of Oxystelma esculentum proved its strong antioxidant activity and can be used as a natural antioxidant.

Submitted: January 6, 2008 · Accepted: March 4, 2008 · Published: May 20, 2008

Recommended Citation

Ashokkumar, Durairaj; Mazumder, Upal Kanti; Gupta, Malaya; Senthilkumar, GP; and Selvan, Vaiyapuri Thamil (2008) "Evaluation of Antioxidant and Free Radical Scavenging Activities of Oxystelma esculentum in Various In Vitro Models," Journal of Complementary and Integrative Medicine: Vol. 5 : Iss. 1, Article 9.
Available at: http://www.bepress.com/jcim/vol5/iss1/9

 
 
 
 

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