Anti-Adipogenic Activity of Capsicum annum (Solanaceae) in 3T3 L1

Abstract

Various extracts of Capsicum annum L were screened for their cytotoxicity and antiadipogenic activity in vitro. In cytotoxic studies all the extracts were screened against the 3T3 L1 cell line. Micro titre tetrazolium (MTT) and sulpho rhodamine B (SRB) assays against the 3T3 L1 cell line determined cytotoxicity. Methanol extract of fruit showed higher cytotoxicity with a lower CTC50 value of 167.46 µg/ml and the remaining extracts show CTC50 ranging from 180-282 µg/ml. In 3T3 L1 cells dexamethazone, 3- isobutyl-1-methyl xanthine (IBMX) and insulin, induced adipogenesis. All the four extracts (methanolic and water extracts of whole plant and fruit) were screened for their antiadipogenic activity in 3T3 L1 cell line; the methanolic fruit extract showed good antiadipogenic activity at 100 µg/ml.

Submitted: May 31, 2008 · Accepted: September 12, 2008 · Published: October 10, 2008

Recommended Citation

M, Arumugam; P, Vijayan; C, Raghu; G, Ashok; SA, Dhanaraj; and CT, Kumarappan (2008) "Anti-Adipogenic Activity of Capsicum annum (Solanaceae) in 3T3 L1," Journal of Complementary and Integrative Medicine: Vol. 5 : Iss. 1, Article 28.
DOI: 10.2202/1553-3840.1151
Available at: http://www.bepress.com/jcim/vol5/iss1/28