Antiangiogenic Effects of Chinese Medicines (Hachimijiogan and Kogikujiogan) on Vascular Endothelial Growth Factor-A-Induced Tube Formation in Human Umbilical Vein Endothelial Cells Co-Cultured with Fibroblasts

Abstract

The aim of this study was to examine the antiangiogenic properties and antioxidant activities of two Chinese medicines (Hachimijiogan and Kogikujiogan). Each of these medicines concentration-dependently inhibited vascular endothelial growth factor-A (VEGF-A)-induced tube formation in a co-culture of human umbilical vein endothelial cells (HUVECs) and fibroblasts. In addition, they each at 100 μg/ml inhibited the VEGF-A-induced cell proliferation of HUVECs. Hachimijiogan at 10 and 100 μg/ml and Kogikujiogan at 100 μg/ml also inhibited the VEGF-A-induced cell migration of HUVECs, and Hachimijiogan at 50 μg/ml and Kogikujiogan at 5, 50, and 100 μg/ml inhibited lipid peroxidation in mouse forebrain homogenates. These findings indicate that Hachimijiogan and Kogikujiogan have antiangiogenic and antioxidant effects, however, the contribution of their antioxidant property towards the observed anti-angiogenic effects requires further investigation.

Submitted: June 2, 2008 · Accepted: July 14, 2008 · Published: August 25, 2008

Recommended Citation

Uchibayashi, Rumi; Yokoyama, Yuri; Nakajima, Yoshimi; Matsunaga, Nozomu; Shimazawa, Masamitsu; and Hara, Hideaki (2008) "Antiangiogenic Effects of Chinese Medicines (Hachimijiogan and Kogikujiogan) on Vascular Endothelial Growth Factor-A-Induced Tube Formation in Human Umbilical Vein Endothelial Cells Co-Cultured with Fibroblasts," Journal of Complementary and Integrative Medicine: Vol. 5 : Iss. 1, Article 20.
Available at: http://www.bepress.com/jcim/vol5/iss1/20

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