Protective Effects of Silymarin against Free Radical-Induced Erythrocyte Lysis

Gholamreza Karimi, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mohammad Hassanzadeh, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Soghra Mehri, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

The oxidative hemolysis of rat erythrocytes induced by 2,2’-azobis–(2-amidinopropane) (AAPH) and its inhibition by silymarin studied. Different concentrations of silymarin showed no significant hemolysis compared with phosphate buffer solution. AAPH (25 mM and 50 mM) induced hemolysis in a time-dependent manner. Silymarin inhibited AAPH (25 mM)-induced hemolysis concentration-dependently. However, in the presence of 50 mM of AAPH, only the two higher concentrations of silymarin inhibited hemolysis. Addition of silymarin 3h after incubation with AAPH (25 mM), had no significant effects on the time course of cell lysis. It is concluded that, in addition to its well-established antioxidant effects, silymarin displays cytoprotective properties.

Submitted: April 5, 2006 · Accepted: September 29, 2006 · Published: December 4, 2006

Recommended Citation

Karimi, Gholamreza; Hassanzadeh, Mohammad; and Mehri, Soghra (2006) "Protective Effects of Silymarin against Free Radical-Induced Erythrocyte Lysis," Journal of Complementary and Integrative Medicine: Vol. 3 : Iss. 1, Article 8.
DOI: 10.2202/1553-3840.1054
Available at: http://www.bepress.com/jcim/vol3/iss1/8

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