Abstract

The effects of sucrose concentration, pH, temperature and aeration rate on the production of biomass and inulinase (2,1-beta-D-fructan fructanohydrolase, EC 3.2.1.7) were investigated using fed-batch fermentation by Kluyveromyces marxianus var. bulgaricus. The best sucrose concentration for enzyme activity was 10 g L-1, whereas activity was inhibited when the sucrose concentration was 20 g L-1. Enzyme activity was strongly influenced by pH of the culture. pH 5.0 was the most suitable for both enzyme activity and cell growth, achieving 98.04 U mL-1 and 14.05 g L-1, respectively, after 72 hours of cultivation. The highest production of inulinase and biomass was observed at temperatures of 25^oC and 30^oC, whereas a decrease in activity was observed at 35^oC and 40^oC. The best aeration rate was 1.0 vvm. The increase in aeration rate or oxygen injection provided no increase in enzyme activity and biomass.

Submitted: November 1, 2006 · Accepted: October 30, 2007 · Published: January 17, 2008

Recommended Citation

Cazetta, Marcia Luciana; Monti, Rubens; and Contiero, Jonas (2008) "Effect of Conditioning on the Production of Inulinase by Kluyveromyces marxianus var. bulgaricus through Fed-Batch Fermentation," International Journal of Food Engineering: Vol. 4 : Iss. 1, Article 5.
Available at: http://www.bepress.com/ijfe/vol4/iss1/art5