Abstract

Based on a three level Box Behnken design involving the variables pH (X1), incubation temperature (X2) and fermentation time (X3), a response surface methodology for the production of carotenoid by a mutant Aspergillus carbonarius CFTRI-UV10046 was standardized. Data analyzed using a second order polynomial equation resulted in the optimized process condition of 46.7 h growth at 29.30C in a corn flour medium of pH 3.3 for carotenoid production. Under these conditions the model predicted 1283.00 µg of carotenoid /g of dry fungal biomass which is 52.19% higher then the initial medium. With regard to biomass, 2.44 g dry weight/100 ml culture medium was obtained after 56 h of growth at 29.70C in the medium of pH-3.6.

Submitted: September 25, 2007 · Accepted: October 23, 2007 · Published: November 3, 2007

Recommended Citation

Sanjay, K. R.; Kumaresan, N.; Manohar, B.; Kumar, S. Umesh; and Vijayalakshmi, G. (2007) "Optimization of Carotenoid Production by Aspergillus Carbonarius in Submerged Fermentation Using a Response Surface Methodology," International Journal of Food Engineering: Vol. 3 : Iss. 5, Article 19.
DOI: 10.2202/1556-3758.1295
Available at: http://www.bepress.com/ijfe/vol3/iss5/art19