Relation between Respirometric Data and Amylolytic Enzyme Production by SSF in Column-Type Bioreactor

Michele Rigon Spier, Federal University of Parana
Ursula Konietzny Greiner, Federal University of Parana
Ralf Greiner, Federal University of Parana
Jose Angel Rodriguez-León, Federal University of Parana
Julio Cesar Carvalho, Federal University of Parana
Adenise Lorenci Woiciechowski, Federal University of Parana
Carlos Ricardo Soccol, Federal University of Parana

Abstract

The use of agro industrial by-products like cassava starch and sugar cane bagasse show great potential as substrates and support amylases production by solid-substrate fermentation (SSF). The production of amylolytic enzymes by Aspergillus niger LPB 28 increased with forced air using a column fermentation system and optimized conditions in a column bioreactor when it was followed by kinetics of respiratory metabolism. The O₂ consumption and CO₂ production by A. niger were measured in the top of the columns through the use of a GC system linked to a program for chromatograph control and integration with 2m length, with helium as the carrier gas and a thermal conductivity detector. The kinetic parameters were determined using the program FERSOL.

Recommended Citation

Spier, Michele Rigon; Greiner, Ursula Konietzny; Greiner, Ralf; Rodriguez-León, Jose Angel; Carvalho, Julio Cesar; Woiciechowski, Adenise Lorenci; and Soccol, Carlos Ricardo (2007) "Relation between Respirometric Data and Amylolytic Enzyme Production by SSF in Column-Type Bioreactor," International Journal of Chemical Reactor Engineering: Vol. 5: A67.
Available at: http://www.bepress.com/ijcre/vol5/A67

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